Ethidium bromide is a nucleic acid stain. It fluoresces under ultraviolet light, especially when bound to double-stranded DNA. It is also a strong mutagen and a possible carcinogen, so must be managed correctly.
Aqueous solutions of ethidium bromide with concentrations less than 10 µg/L (10 ppb) are not considered hazardous and may be discharged to the sewer. Solutions containing greater than 10 µg/L (10 ppb) must be chemically treated or collected as hazardous waste.
We recommend GreenBags for treating dilute (over 10 µg/L) solutions of ethidium bromide. The treated water can be disposed of in the sink. The tea bags, which contain activated carbon, absorb the ethidium bromide so tightly that it is safe to put them in the trash.
We give a starter kit of three tea bags to each laboratory. Contact Environmental Programs to request them. You can buy more GreenBags from VWR via eProcurement. The product number is 100371-168, and a pack of 25 teabags costs about $90.
Gels that contain ethidium bromide may be doubled-bagged, labeled "non-hazardous" and placed in the trash if they contain less than 0.1 percent ethidium bromide. If the gel is pink or red, the ethidium concentration is higher than 0.1 percent. In this case, the gel is handled as hazardous waste.
Items contaminated with concentrated ethidium bromide are managed as hazardous waste. Pipette tips contaminated with dilute ethidium bromide are dried and placed into a lab glass bin. Other items contaminated with dilute ethidium bromide may be double-bagged, labeled "non-hazardous," and placed in the trash. Please double-bag to keep waste handlers from being exposed.